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dapi solution (1 thermofisher scientific #62248)  (Thermo Fisher)


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    Thermo Fisher dapi solution (1 thermofisher scientific #62248)
    Dapi Solution (1 Thermofisher Scientific #62248), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+solution+thermofisher+62248/dapi+solution/pmc11621072-56-8-12
    Average 90 stars, based on 1 article reviews
    dapi solution (1 thermofisher scientific #62248) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Defective CFTR modulates mechanosensitive channels TRPV4 and PIEZO1 and drives endothelial barrier failure.
    Article Snippet: iScience Article ll OPEN ACCESS Defective CFTR modulates mechanosensitive channels TRPV4 and PIEZO1 and drives endothelial barrier failure A B F C DE G H I K L J Jean-Pierre Amoakon, Jesun Lee, Pramodha Liyanage, ..., Goutham Mylavarapu, Raouf Amin, Anjaparavanda P. Naren. amoakoja@mail.uc.edu (J.-P.A.) apnaren@cshs.org (A.P.N.). Highlights CFTR deficiency promotes barrier failure in the pulmonary microvascular endothelium CFTR deficiency alters endothelial cells lipid metabolism and membrane fluidity CFTR deficiency sensitizes TRPV4 to fluid shear stress CFTR directly interacts with PIEZO1 Amoakon et al., iScience 27, 110703 September 20, 2024 a 2024 The Author(s).. Published by Elsevier Inc. https://doi.org/10.1016/ j.isci.2024.110703 OPEN ACCESS iScience ll

    Article Title: Defective CFTR modulates mechanosensitive channels TRPV4 and PIEZO1 and drives endothelial barrier failure
    Article Snippet: The next day, samples were washed 3 times for 10 min each on side-to-side shaker with PBS + 0.05% Tween-20 (Sigma, Cat#P9416-100ML) before being incubated with Alexa Fluor 488 Goat anti-Rabbit IgG antibody (Invitrogen, Cat#A-11008) diluted 1:1000 in PBS, for 1h at RT. .. Samples were then washed 3 times for 10 min each on side-to-side shaker with PBS + 0.05% Tween-20 and incubated with DAPI solution (ThermoFisher, Cat62248) diluted 1:1000 in PBS, for 5 min at RT. .. Samples were mounted in vectashield antifade mounting media (Vector Laboratories, Cat#H-1000) and imaging was performed using a fluorescence microscope (Olympus FV1200).



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